The instrument model：inVia Reflex
This system is a compact automated laser Raman microscope which is very user–friendly. Switching between excitation lines is simple and alignment of the lasers and optics is operated via computer controlled software.
The collection optics are based on a Leica DMLM microscope. Samples are placed underneath a microscope objective (×5, 20, 50) and are excited by the laser directed through the microscope. The scattered light is collected (180° backscattering geometry) along the same optical path as The incoming laser.
Raman scattered light passes through a notch filter (used as one of the reflective elements for the incident laser beam) through a grating (2400 or 1200 mm/groove) that disperses the scattered light over a CCD array detector.
A computer-controlled 3-axis motorised stage with auto focus abilities allows 3-D mapping with 1-5 μm spatial resolution. The instrument operates over five laser lines 488, 514, 633, 785 and 830 nm-the 785 and 830 nm are particularly useful for the analysis of biological samples.